Primer probe combination and kit for detecting feline herpesvirus type 1 based on real-time MIRA and application of primer probe combination and kit

The invention relates to a real-time MIRA-based primer probe combination and kit for detecting feline herpesvirus type 1 and application thereof, the primer probe combination comprises an upstream primer, a downstream primer and a probe, the upstream primer is selected from one of the sequences show...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	WU XIUJUAN, YANG DEQUAN, LIU JIAN, LI XIN, CHEN XINGHAO, TANG CONGSHENG, SHEN HAIXIAO, WANG XIAOXU, YANG XIANCHAO, WANG JIAN, SHENG WENWEI, GE FEIFEI, CHEN QI, JU HOUBIN, ZHAO HONGJIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to a real-time MIRA-based primer probe combination and kit for detecting feline herpesvirus type 1 and application thereof, the primer probe combination comprises an upstream primer, a downstream primer and a probe, the upstream primer is selected from one of the sequences shown in SEQ ID NO: 1-SEQ ID NO: 3, the downstream primer is selected from one of the sequences shown in SEQ ID NO: 4-SEQ ID NO: 6, the sequence of the probe is shown in SEQ ID NO: 7, and the probe is selected from one of the sequences shown in SEQ ID NO: 4-SEQ ID NO: 6. According to the kit adopting the primer probe combination, a reaction system is constructed, the optimal reaction condition and the optimal primer pair are determined, a method for detecting the feline herpesvirus type 1 is established and optimized, and the detection method is high in sensitivity, good in specificity, simple to operate, high in detection speed, low in cost and high in detection efficiency. The kit has an important application value i