Fritillaria cirrhosa and fritillaria ussuriensis detection and distinguishing method and device and terminal equipment

The invention is suitable for the technical field of nucleic acid detection, and provides a bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis detection and distinguishing method and device and terminal device.The detection and distinguishing method comprises the steps that according...

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Hauptverfasser:	YANG YANCHAO, ZHANG XUENING, LIU BEIJIA, SHI MEINA, ZOU WEI, LIN, WAN-JIUN, MA WENZHE, YANG DONGFAN, HUANG ZIFENG, LANG SONGPING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention is suitable for the technical field of nucleic acid detection, and provides a bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis detection and distinguishing method and device and terminal device.The detection and distinguishing method comprises the steps that according to a PAM sequence corresponding to Cas protein, at least one pair of guide sequences is screened from specific DNA fragments of bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis; generating crRNA corresponding to each pair of guide sequences according to each pair of guide sequences and a protein anchoring sequence corresponding to the Cas protein; according to the detection contrast ratio of each pair of crRNA to the specific DNA fragments of the bulbus fritillariae cirrhosae and the bulbus fritillariae ussuriensis, one pair of crRNA is screened out; according to the crRNA, the Cas protein and a to-be-tested sample, combining a test probe to generate a test result, and distinguishing bulbus fritill