Preparation method of microbial exovesicles

The invention discloses a preparation method of microbial exovesicles. The method comprises the following steps: centrifuging a microbial culture solution at a low temperature, collecting thallus precipitates, treating the thallus precipitates with cell walls to remove the cell walls, centrifuging, collecting the precipitates to obtain protoplast precipitates, washing the protoplast precipitates or thallus precipitates without the cell walls with PBS, resuspending, and extruding the protoplast precipitates or the thallus precipitates with the cell walls by using a liposome extruder to obtain the liposome. And extruding through a series of polycarbonate nuclear pore membranes with different pore diameters in sequence to obtain the microbial external vesicles. According to the method, the extrusion method is used for preparing the microbial outer vesicles for the first time, the preparation method is simple and easy to operate, and compared with a traditional ultracentrifugation method, the prepared outer vesic