Multi-flux real-time electric monitoring heart chip and manufacturing method thereof

The invention belongs to the technical field of heart chips, and particularly relates to a multi-flux real-time electric monitoring heart chip and a manufacturing method thereof.The multi-flux real-time electric monitoring heart chip comprises a perfusion layer, a culture room layer and a glass substrate-electrode plate which are fixedly connected in sequence from top to bottom; a tree-shaped step perfusion channel is formed in the perfusion layer, the tree-shaped step perfusion channel comprises n stages of mixing flow channel groups which are sequentially arranged in a communicating mode in the liquid flowing direction, the liquid outlet end of the mixing flow channel group located at the tail end communicates with the liquid inlet end of a liquid injection part, and the liquid outlet end of the liquid injection part communicates with an outlet; the culture room layer is provided with a plurality of culture parts, the culture parts are in one-to-one correspondence with the liquid injection parts, and the li