Cydia pomonella lethal gene synthesized in vitro and application thereof

The invention belongs to the technical field of grain storage, and particularly relates to a cydia pomonella lethal gene synthesized in vitro and application thereof.The cydia pomonella lethal gene is obtained by utilizing a known Snf7 gene of potato beetles, obtaining a homologous gene RdSnf7 of cy...

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Bibliographische Detailangaben
Hauptverfasser:	WANG KANGXU, XU HONGLEI, TANG PEI'AN, WU XUEYOU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of grain storage, and particularly relates to a cydia pomonella lethal gene synthesized in vitro and application thereof.The cydia pomonella lethal gene is obtained by utilizing a known Snf7 gene of potato beetles, obtaining a homologous gene RdSnf7 of cydia pomonella through transcriptome database comparison and synthesizing dsRdSnf7 according to RdSnf7 in vitro transcription; the invention aims to screen the Snf7 homologous gene of the gotacross beetle and verify the lethal effect of the gene, explore the influence of phosphine resistance on the RNAi effect under the condition of proving the lethal effect, and further explore the relationship between the dsRNA concentration and the lethal effect; the stability of the dsRNA in a grain storage environment is preliminarily explored. 本发明属于粮食储藏技术领域，具体涉及一种体外合成的谷蠧致死基因及其应用，所述谷蠧致死基因是利用已知马铃薯甲虫的Snf7基因，通过转录组数据库比对得到谷蠹的同源基因RdSnf7，根据RdSnf7体外转录合成dsRdSnf7；本发明旨在筛选谷蠹的Snf7同源基因并验证其致死效果，在证明致死效果的情况下，探究磷化氢抗性对RNAi效果的影响，并进一步探究dsRNA浓度与致死效果