4-vinyl guaiacol oxygenase mutant, genetically engineered bacterium and application of 4-vinyl guaiacol oxygenase mutant and genetically engineered bacterium

The invention provides a 4-vinyl guaiacol oxygenase mutant, a genetically engineered bacterium and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. According to the 4-vinyl guaiacol oxygenase disclosed by the invention, a central site of a pocket of the 4-vinyl guaiacol oxygenase Iso is mutated, and the enzyme activity of mutants T216M, T216L, S280M, G307P and M351F is 1.04-2.29 times that of mutants before mutation. The substrate concentration is 100 mmol/L, the conversion rate of the vanillin is 67.9%-88.44%, and the yield reaches 10.32-13.44 g/L. The Iso mutant provided by the invention can be used for efficiently catalyzing 4-vinyl guaiacol to generate vanillin, and has a good industrial application prospect. 本发明提供了4-乙烯基愈创木酚加氧酶突变体和基因工程菌及其应用，属于基因工程技术领域。本发明对4-乙烯基愈创木酚加氧酶Iso的口袋中心位点进行突变，其突变体T216M、T216L、S280M、G307P、M351F的酶活是突变之前的1.04-2.29倍。100mmol/L底物浓度，香兰素转化率为67.9％-88.44％，产量达10.32-13.44g/L。利用本发明提供的Iso突变体能够高效催化4-乙烯基愈创木酚生成香兰素，具有良好的工业应用前景。