Application of protein NT5C1B in porcine sperm in-vitro cryopreservation

The invention provides an application of a protein NT5C1B in in-vitro cryopreservation of porcine sperms. The freezing preservation quality of the boar semen is improved by adding the antifreeze protein NT5C1B into the semen freezing preservation liquid. According to the semen diluent provided by the invention, the cryopreservation activity and quality of semen can be remarkably improved, and the in-vitro preservation time of sperms can be prolonged; meanwhile, compared with a control group, the unfrozen sperm motility can be improved by about 11%. The method is easy and convenient to operate, remarkable in effect and free of any harmful effect on pigs. The method can be widely applied to pig semen preservation and related in-vitro embryo production, embryo transfer industry and embryo engineering, and has a wide market prospect. 本发明提供蛋白NT5C1B在猪精子体外冷冻保存中的应用。通过向精液冷冻保存液中添加抗冻蛋白NT5C1B来提高猪精液冷冻保存品质。本发明提供的精液稀释液能够显著提高精液冷冻保存活力和质量，延长精子体外保存时间；同时，解冻后精子活力与对照组相比能提高11％左右。本发明操作简便，效果显著，对猪不产生任何有害影响。可以在猪精液保存及相关的体外胚胎生产、胚胎移植产业和胚胎