Method for producing 2-keto-D-gluconic acid through double-enzyme catalysis

The invention relates to the technical field of microbial fermentation production, in particular to a method for producing 2-keto-D-gluconic acid through double-enzyme catalysis. The invention relates to a method for producing 2-keto-D-gluconic acid through double-enzyme catalysis, which comprises the following steps: taking corn starch hydrolyzed sugar as a substrate, adding a glucose dehydrogenase treating fluid and a gluconic acid dehydrogenase treating fluid into the corn starch hydrolyzed sugar, and reacting for 24-48 hours to obtain the 2-keto-D-gluconic acid. The method for producing 2-keto-D-gluconic acid through double-enzyme catalysis has the advantages of being high in raw material utilization rate and high in production intensity. 本申请涉及微生物发酵生产的技术领域，更具体地说，它涉及一种双酶催化生产2-酮基-D-葡萄糖酸的方法。一种双酶催化生产2-酮基-D-葡萄糖酸的方法，其步骤为：以玉米淀粉水解糖为底物，向玉米淀粉水解糖中添加葡萄糖脱氢酶处理液及葡萄糖酸脱氢酶处理液，反应24-48h，得到2-酮基-D-葡萄糖酸。本申请的双酶催化生产2-酮基-D-葡萄糖酸的方法具有原料利用率高，生产强度高的优点。