Method for improving yield of L-valine through mutated bamDM gene

The invention is applicable to the technical field of bioengineering, and provides a method for improving the yield of L-valine through a mutated bamDM gene, the mutant gene bamDM with the nucleotide sequence shown as SEQ ID NO.1 is used for replacing the BamD gene of escherichia coli ATCC 8739 to obtain escherichia coli (Escherichia coli) DXMC014, the preservation number is CCTCC NO: M2024590, the preservation date is Wuhan University, Wuhan, China, on April 1, 2024, and the preservation number is CCTCC NO: M2024590. And China Center for Type Culture Collection. In conclusion, the Escherichia coli DXMC014 constructed by the invention improves the fermentation acid production level of the L-valine, and the growth speed and the biomass are both improved compared with those of an original strain. 本发明适用于生物工程技术领域，提供了一种通过突变后的bamDM基因提高L-缬氨酸产量的方法，使用突变基因bamDM，核苷酸序列如SEQ ID NO.1所示，替换大肠杆菌ATCC 8739的BamD基因，得到大肠杆菌（Escherichia coli）DXMC014，其保藏编号：CCTCC NO:M2024590，保藏日期：2024年4月1日，保藏地址：中国，武汉，武汉大学，中国典型培养物保藏中心。综上，本发明构建的大肠杆菌DXMC0