Modification method for improving affinity of RHD1 and linear ubiquitin chain and retaining specificity of RHD1 and linear ubiquitin chain

The invention belongs to the technical field of biological materials, and particularly discloses a transformation method for improving affinity of RHD1 and a linear ubiquitin chain and retaining specificity of the RHD1. Amino acid of a binding interface of the RHD1 and the linear ubiquitin chain is analyzed by utilizing bioinformatics software based on RHD1 (1-200aa) and linear double ubiquitin structure data; the method comprises the following steps of: selecting important amino acid sites for point mutation or combinatorial mutation, determining whether the mutation improves the affinity of combining with a linear ubiquitin chain or not through enzyme-linked immunosorbent assay (ELISA), developing the RHD1 into a protein ligand capable of specifically identifying and efficiently combining with the linear ubiquitin chain, and providing a powerful tool for specifically identifying the linear ubiquitin chain and researching the function of the linear ubiquitin chain. 本发明属于生物材料技术领域，具体公开了一种提高RHD1与线性泛素链亲和力并保留其特异性