Preparation method of recombinant human octoplasminogen

The invention provides a preparation method of recombinant human octoplasminogen, and belongs to the field of fermentation. The method comprises the following steps: 1) fermentation culture: inoculating a fermentation tank containing a culture medium with a seed solution, activating, replenishing glycerol, stopping replenishing glycerol when the OD600 value of a thallus is 140-180, adding methanol to induce expression when the replenished glycerol is used up, adding a YP solution at the same time, and finishing fermentation after inducing expression for 40-80 hours; and (2) separation and purification: collecting the fermentation supernatant, and separating and purifying to obtain the recombinant plasminogen. By controlling multiple conditions in the fermentation process of the recombinant human Octoplasminogen, the expression quantity of the target product is remarkably increased on the premise that the technological operation is simple and easy to implement, the unexpected technical effect is achieved compa