4-vinyl guaiacol oxygenase mutant for synthesizing vanillin and engineering bacteria of 4-vinyl guaiacol oxygenase mutant

The invention provides a 4-vinyl guaiacol oxygenase mutant for synthesizing vanillin and engineering bacteria of the 4-vinyl guaiacol oxygenase mutant, and belongs to the technical field of genetic engineering. According to the present invention, any two or more of the 27th site, the 156th site, the 216th site and the 351th site of the 4-vinyl guaiacol oxygenase gene Iso are subjected to mutation so as to obtain the 4-vinyl guaiacol oxygenase mutant; the gene engineering strain capable of efficiently catalyzing the ferulic acid to generate the vanillin through a two-step enzyme method is successfully constructed, the vanillin can be catalytically generated within 5 hours in an enzyme reaction system and can be up to 19.58 g/L at most, ferulic acid decarboxylase and 4-vinyl guaiacol oxygenase can still be stably expressed after passing to 1000 generations, the enzyme activity is not influenced, and the method is suitable for industrial production. The industrial production of the natural vanillin is realized,