Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof
The invention provides a genetically engineered RGD peptide exosome drug delivery biological agent as well as preparation and application thereof, and belongs to the technical field of preparation of antitumor drugs. According to the genetically engineered RGD peptide exosome provided by the inventi...
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| creator | WANG MIN SUN YONGPING WANG ZHANGFEI LIU ZHONG WU PEIPEI |
| description | The invention provides a genetically engineered RGD peptide exosome drug delivery biological agent as well as preparation and application thereof, and belongs to the technical field of preparation of antitumor drugs. According to the genetically engineered RGD peptide exosome provided by the invention, RGD peptide and CD63 protein fusion plasmids are electrically transferred into HEK293T cells in a gene manipulation manner, and then the RGD peptide modified exosome in supernatant is obtained. According to the genetically engineered RGD peptide exosome drug delivery biological agent provided by the invention, an anti-cancer drug DOX is enriched into an exosome by adopting an electroporation technology, and the DOX-loaded RGD exosome is obtained after washing. The DOX-loaded RGD exosome provided by the invention can target lung cancer cells, enhance the drug delivery effect, reduce the side effects of drugs and inhibit the growth of tumor cells.
本发明提供了一种基因工程化的RGD肽外泌体药物递送生物制剂及其制备与应用，属于抗肿瘤药物制备技术领域。本发明提供的基因工程化的RGD |
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本发明提供了一种基因工程化的RGD肽外泌体药物递送生物制剂及其制备与应用，属于抗肿瘤药物制备技术领域。本发明提供的基因工程化的RGD</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240723&DB=EPODOC&CC=CN&NR=118373919A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240723&DB=EPODOC&CC=CN&NR=118373919A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG MIN</creatorcontrib><creatorcontrib>SUN YONGPING</creatorcontrib><creatorcontrib>WANG ZHANGFEI</creatorcontrib><creatorcontrib>LIU ZHONG</creatorcontrib><creatorcontrib>WU PEIPEI</creatorcontrib><title>Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof</title><description>The invention provides a genetically engineered RGD peptide exosome drug delivery biological agent as well as preparation and application thereof, and belongs to the technical field of preparation of antitumor drugs. According to the genetically engineered RGD peptide exosome provided by the invention, RGD peptide and CD63 protein fusion plasmids are electrically transferred into HEK293T cells in a gene manipulation manner, and then the RGD peptide modified exosome in supernatant is obtained. According to the genetically engineered RGD peptide exosome drug delivery biological agent provided by the invention, an anti-cancer drug DOX is enriched into an exosome by adopting an electroporation technology, and the DOX-loaded RGD exosome is obtained after washing. The DOX-loaded RGD exosome provided by the invention can target lung cancer cells, enhance the drug delivery effect, reduce the side effects of drugs and inhibit the growth of tumor cells.
本发明提供了一种基因工程化的RGD肽外泌体药物递送生物制剂及其制备与应用，属于抗肿瘤药物制备技术领域。本发明提供的基因工程化的RGD</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjEOgkAURGksjHqH7wEsCIVSGlSsLIw9-bLDssm6-11WI7cXiAewmrzkzWTmybOEQzQ1W9sTnDYOCFB0LQ8kkGgUCB_f-QdIhZcmBWveCD3djbdej01iDReJnSIJEA4cjXcTs4gdlIljOyz7ZpnMGrYdVr9cJOvT8VacNxBfoROux0dVcUnTXbbN8jTfZ_84X8fLRCE</recordid><startdate>20240723</startdate><enddate>20240723</enddate><creator>WANG MIN</creator><creator>SUN YONGPING</creator><creator>WANG ZHANGFEI</creator><creator>LIU ZHONG</creator><creator>WU PEIPEI</creator><scope>EVB</scope></search><sort><creationdate>20240723</creationdate><title>Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof</title><author>WANG MIN ; SUN YONGPING ; WANG ZHANGFEI ; LIU ZHONG ; WU PEIPEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118373919A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG MIN</creatorcontrib><creatorcontrib>SUN YONGPING</creatorcontrib><creatorcontrib>WANG ZHANGFEI</creatorcontrib><creatorcontrib>LIU ZHONG</creatorcontrib><creatorcontrib>WU PEIPEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG MIN</au><au>SUN YONGPING</au><au>WANG ZHANGFEI</au><au>LIU ZHONG</au><au>WU PEIPEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof</title><date>2024-07-23</date><risdate>2024</risdate><abstract>The invention provides a genetically engineered RGD peptide exosome drug delivery biological agent as well as preparation and application thereof, and belongs to the technical field of preparation of antitumor drugs. According to the genetically engineered RGD peptide exosome provided by the invention, RGD peptide and CD63 protein fusion plasmids are electrically transferred into HEK293T cells in a gene manipulation manner, and then the RGD peptide modified exosome in supernatant is obtained. According to the genetically engineered RGD peptide exosome drug delivery biological agent provided by the invention, an anti-cancer drug DOX is enriched into an exosome by adopting an electroporation technology, and the DOX-loaded RGD exosome is obtained after washing. The DOX-loaded RGD exosome provided by the invention can target lung cancer cells, enhance the drug delivery effect, reduce the side effects of drugs and inhibit the growth of tumor cells.
本发明提供了一种基因工程化的RGD肽外泌体药物递送生物制剂及其制备与应用，属于抗肿瘤药物制备技术领域。本发明提供的基因工程化的RGD</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof |
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