Genetically engineered RGD peptide exosome drug delivery biological agent and preparation and application thereof

The invention provides a genetically engineered RGD peptide exosome drug delivery biological agent as well as preparation and application thereof, and belongs to the technical field of preparation of antitumor drugs. According to the genetically engineered RGD peptide exosome provided by the invention, RGD peptide and CD63 protein fusion plasmids are electrically transferred into HEK293T cells in a gene manipulation manner, and then the RGD peptide modified exosome in supernatant is obtained. According to the genetically engineered RGD peptide exosome drug delivery biological agent provided by the invention, an anti-cancer drug DOX is enriched into an exosome by adopting an electroporation technology, and the DOX-loaded RGD exosome is obtained after washing. The DOX-loaded RGD exosome provided by the invention can target lung cancer cells, enhance the drug delivery effect, reduce the side effects of drugs and inhibit the growth of tumor cells. 本发明提供了一种基因工程化的RGD肽外泌体药物递送生物制剂及其制备与应用，属于抗肿瘤药物制备技术领域。本发明提供的基因工程化的RGD