Preparation method of decellularized biological net membrane and decellularized biological net membrane prepared by same

The invention discloses a preparation method of a decellularized biological omentum and the decellularized biological omentum prepared by the preparation method. The preparation method comprises the following steps: 1) taking fresh omentum majus tissue for degreasing treatment; 2) soaking the omentum majus tissue without fat in a decellularization solution I in an oscillating manner for treatment; 3) treating the omentum majus tissue treated by the decellularization liquid I in a decellularization liquid II; the method comprises the following steps of 1, preparing a decellularized solution I, 2, preparing a decellularized solution I, 3, preparing a decellularized solution II, 4, treating the omentum majus tissue treated by the decellularized solution II in a decellularized solution III, and 5, freeze-drying and sterilizing the omentum majus tissue treated by the decellularized solution III. The method is simpler and more convenient to operate and shorter in period, and the DNA content in the decellularized om