Method for detecting RNF180 gene promoter methylation based on nanopore targeted sequencing technology and detection kit

The invention discloses a method for detecting RNF180 gene promoter methylation based on a nanopore targeted sequencing technology and a detection kit, and belongs to the technical field of molecular medicine. The methylation state of a methylation site of the RNF180 gene promoter is detected by combining a nanopore length reading sequencing technology and a Cas9 in-vitro cutting technology, four crRNA spacer sequences for cutting the RNF180 gene promoter in a targeted manner through Cas9 are designed for an RNF180 promoter region, cutting is performed twice on the upstream and downstream of a target region respectively, and the RNF180 promoter region and a flanking region thereof are included. According to the method for detecting the methylation sites, a sample does not need to be treated through bisulfite, the most original methylation state of sample DNA can be rapidly and efficiently obtained, the methylation level can be evaluated from the overall level, and the methylation state of a single methylation