Seed property recovery method of subculture degenerated black fungus strain and application
The invention provides a sexual restoration method for subculture degenerated black fungus strains and application, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: inoculating an activated subculture degenerated black fungus strain into a ba...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a sexual restoration method for subculture degenerated black fungus strains and application, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: inoculating an activated subculture degenerated black fungus strain into a barren wood chip culture medium for culturing at 18-20 DEG C, inoculating cultured hypha into a barren PDA culture medium for culturing at 20-22 DEG C, inoculating the cultured hypha into a wood chip culture medium for culturing at 22-25 DEG C, and inoculating the cultured hypha into a PDA culture medium for culturing at 22-25 DEG C; and inoculating the cultured hyphae into a conventional wood chip culture medium for culturing at 25-28 DEG C, and inoculating the obtained hyphae into a cPDA culture medium for culturing at 25-28 DEG C to obtain the black fungus strain with recovered species nature. According to the method disclosed by the invention, the species nature of the degenerated black fungus strain caused by mu |
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