Seed property recovery method of subculture degenerated black fungus strain and application

The invention provides a sexual restoration method for subculture degenerated black fungus strains and application, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: inoculating an activated subculture degenerated black fungus strain into a ba...

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Hauptverfasser: HAN CHUANG, YU DESHUI, ZHANG JIECHI, ZHANG XIUFENG, ZHOU SHUYANG, ZHANG SHIMENG, TIAN SHUANG, DAI XIAODONG, ZHU JIANAN, WANG YUXIA, MA YINPENG, ZHANG PIQI, SHANG LIANG, ZHEN TAO, WANG XIAOYONG, GUO YAN, LIU JIANING, MA QINGFANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a sexual restoration method for subculture degenerated black fungus strains and application, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: inoculating an activated subculture degenerated black fungus strain into a barren wood chip culture medium for culturing at 18-20 DEG C, inoculating cultured hypha into a barren PDA culture medium for culturing at 20-22 DEG C, inoculating the cultured hypha into a wood chip culture medium for culturing at 22-25 DEG C, and inoculating the cultured hypha into a PDA culture medium for culturing at 22-25 DEG C; and inoculating the cultured hyphae into a conventional wood chip culture medium for culturing at 25-28 DEG C, and inoculating the obtained hyphae into a cPDA culture medium for culturing at 25-28 DEG C to obtain the black fungus strain with recovered species nature. According to the method disclosed by the invention, the species nature of the degenerated black fungus strain caused by mu