Quality control method of herba aristolochiae sample
The invention discloses a quality control method of a herba aristolochiae sample. The quality control method comprises the following steps: (1) preparing a reference solution and a test solution; caffeic acid, caffeoyl malic acid, ferulic acid and rutin are used as reference substances of reference...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a quality control method of a herba aristolochiae sample. The quality control method comprises the following steps: (1) preparing a reference solution and a test solution; caffeic acid, caffeoyl malic acid, ferulic acid and rutin are used as reference substances of reference substances, and reference substance solutions of the reference substances are prepared respectively; (2) respectively sucking the reference substance solution and the test solution for ultra-high performance liquid chromatography analysis, and constructing a characteristic spectrum of the sample to be detected, the characteristic peaks including four characteristic peaks respectively corresponding to a caffeic acid reference substance peak, a caffeoyl malic acid reference substance peak, a ferulic acid reference substance peak and a rutin reference substance peak; and (3) carrying out qualitative analysis and quantitative analysis. The method is easy to operate, methodology verifies that the reproducibility and sta |
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