Quality control method of herba aristolochiae sample

The invention discloses a quality control method of a herba aristolochiae sample. The quality control method comprises the following steps: (1) preparing a reference solution and a test solution; caffeic acid, caffeoyl malic acid, ferulic acid and rutin are used as reference substances of reference...

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Hauptverfasser: YUAN JIAN, ZHAI YANJUAN, CHEN SHENGJUN, WANG XIEHE, ZHU QIANQIAN, LI SONG, ZHU ZICONG, ZHOU HAIQIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a quality control method of a herba aristolochiae sample. The quality control method comprises the following steps: (1) preparing a reference solution and a test solution; caffeic acid, caffeoyl malic acid, ferulic acid and rutin are used as reference substances of reference substances, and reference substance solutions of the reference substances are prepared respectively; (2) respectively sucking the reference substance solution and the test solution for ultra-high performance liquid chromatography analysis, and constructing a characteristic spectrum of the sample to be detected, the characteristic peaks including four characteristic peaks respectively corresponding to a caffeic acid reference substance peak, a caffeoyl malic acid reference substance peak, a ferulic acid reference substance peak and a rutin reference substance peak; and (3) carrying out qualitative analysis and quantitative analysis. The method is easy to operate, methodology verifies that the reproducibility and sta