Method for acquiring three-dimensional fluorescence image of tobacco

The invention discloses a method for acquiring a three-dimensional fluorescence image of tobacco, which comprises the following steps: grinding a tobacco sample, and preparing the ground tobacco sample into a sample solution; preparing a target sample solution meeting an absorbance standard based on the sample solution; performing fluorescence testing on the target sample solution to obtain the fluorescence intensity of the target sample solution; and connecting the points with the same fluorescence intensity to form a contour map, and taking the contour map as a three-dimensional fluorescence map. According to the method, on the basis of preparing an ideal tobacco sample, fluorescence detection can be carried out on the tobacco sample meeting the absorbance standard, and a reliable and accurate three-dimensional fluorescence map is obtained. 本发明公开了一种烟草三维荧光图像的获取方法，包括：将烟草样品磨碎，将磨碎后的烟草样品制作成样品溶液；基于样品溶液，制备满足吸光度标准的目标样品溶液；对目标样品溶液进行荧光测试，得到目标样品溶液的荧光强度；连接荧光强度相等的点，形成等高线图，将所述等高线图作为三维荧光图。本发明能够在制备出理想烟草样品的基础上，对满足吸光度标准的烟草样品进