Method for collecting zebra fish urine metabolites

The invention relates to the technical field of biological metabolite collection, and discloses a zebra fish urine metabolite collection method. The zebra fish urine metabolite collection method comprises the general steps that S1, the water temperature in a fish tank is constantly set to be 18 DEG C; s2, starting a constant-flow injection pump, and setting the flow rate to be 1ml/h; s3, collecting zebra fish urine metabolite for 24 hours at the speed of v = 1ml/h, and putting the zebra fish urine metabolite into an ice-water bath; s4, concentrating the urine sample, namely concentrating 24 ml of collected zebra fish urine metabolite to 1 ml through reduced pressure rotary evaporation in an ice-water bath; s5, centrifuging the urine sample, and setting the rotating speed to be 3500r/min for 4 minutes; the filter is used for removing impurities in samples; and S6, storing the sample in a refrigerator at the temperature of-80 DEG C for later use. It can be guaranteed to the maximum extent that metabolites colle