Duplex nucleic acid detection kit and detection method for talaromyces marneffei and pneumocystis

The invention relates to a talaromyces marneffei and pneumocystis duplex nucleic acid detection kit and a detection method. Specific primers and probes are designed for talaromyces marneffei and pneumocystis respectively; polymerase chain reaction and a fluorescent probe technology are adopted to carry out amplification detection on high-conservative-specificity sequences of talaromyces marneffei and pneumocystis respectively, and auxiliary diagnosis of infection of talaromyces marneffei and pneumocystis is carried out. The detection method can realize qualitative detection of the same sample, and has the advantages of strong specificity, high accuracy and high detection efficiency. 本发明涉及一种马尔尼菲篮状菌、肺孢子菌二联核酸检测试剂盒及检测方法，分别针对马尔尼菲篮状菌、肺孢子菌设计特异性的引物和探针，采用聚合酶链式反应及荧光探针技术分别对马尔尼菲篮状菌、肺孢子菌高保守特异性的序列进行扩增检测，辅助诊断马尔尼菲篮状菌、肺孢子菌的感染。该检测方法能够实现对同一样本的定性检测，特异性强，准确率高，且检测效率高。