NGS method containing poly (A) tail plasmid sequencing and application of NGS method

The invention provides a poly (A)-containing tail plasmid sequencing NGS method and application thereof, and the NGS method comprises the following steps: carrying out breaking reaction on plasmid DNA to be detected by adopting a breaking terminal reaction system under the conditions that the hot cover temperature is 70 +/-2 DEG C, the reaction is carried out for 1-3 minutes at 37 +/-2 DEG C, and the reaction is carried out for 14-16 minutes at 65 +/-2 DEG C; the broken end reaction system comprises a broken reaction buffer solution, plasmid DNA to be detected and a fragmentation enzyme mixed solution; connecting the broken fragment with a linker in a connection reaction system, and constructing a sequencing library in a non-amplification manner; carrying out quality inspection and sequencing on the sequencing library to be tested; and performing result analysis and statistics on the sequencing offline data. The method is simple, convenient, rapid and wide in application, can realize low-sample nucleic acid i