Engineered enzymes and methods of making and using

The present invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (...

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Bibliographische Detailangaben
Hauptverfasser:	SHAH, JAYESH, SHAH ANKUR M, GRIFFIN BENJAMIN M, COLQUITT JAMES R, WALLNER JOHN R, ZHANG DANLU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The present invention provides a non-naturally occurring microbial organism having a 1, 6-hexanediol (HDO) pathway wherein an HDO pathway enzyme is expressed in an amount sufficient to produce 6-aminocaproic acid semialdehyde, HDO, or both. The invention also provides a non-naturally occurring microbial organism having an HMD pathway, wherein an HMD pathway enzyme is expressed in an amount sufficient to produce 6-aminocaproic acid semialdehyde, HMD, or both. The present invention further provides biologically derived HMD, 6-aminocaproic acid semialdehyde and/or HDO as well as methods for the production of biologically derived HMD, 6-aminocaproic acid semialdehyde and/or HDO. 本发明提供了一种