Recombinant Meq gene-deleted Marek's disease virus strain for expressing varIBDV VP2 gene as well as construction method and application of recombinant Meq gene-deleted Marek's disease virus strain

The invention discloses a recombinant Meq gene-deleted Marek's disease virus strain for expressing a varIBDV VP2 gene as well as a construction method and application of the recombinant Meq gene-deleted Marek's disease virus strain. The recombinant Meq gene deleted Marek's disease vir...

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Bibliographische Detailangaben
Hauptverfasser: GAO YULONG, CUI HONGYU, WANG SUYAN, LIU YONGZHEN, DUAN YULU, CHEN YUNTONG, QI XIAOLE, ZHANG YANPING, LIU CHANGJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a recombinant Meq gene-deleted Marek's disease virus strain for expressing a varIBDV VP2 gene as well as a construction method and application of the recombinant Meq gene-deleted Marek's disease virus strain. The recombinant Meq gene deleted Marek's disease virus strain is obtained by inserting a gene segment CMV-VP2 containing a CMV promoter sequence, a varIBDV VP2 gene and a terminator sequence into a Meq gene deleted Marek's disease virus strain rMS [delta] Meq US2 gene and replacing a nucleotide sequence between the 156501 < th > site and the 157116 < th > site of an rMS [delta] Meq genome by using a recombinant cloning technology, and is named as rMDV-varVP2. Researches show that the recombinant Meq gene deleted Marek's disease virus rMDV-varVP2 obtained by the invention has the same in-vitro replication ability as a parent virus rMS delta Meq strain and good genetic stability, and after chicken is immunized by the rMDV-varVP2, a good protection effect can be provided for IBDV var