Spatial resolution type hepatic fibrosis chip and use method thereof

The invention discloses a spatial resolution type hepatic fibrosis chip and a use method thereof, the internal space of the chip is divided into a plurality of chambers, the bottom of each chamber partition is provided with a micro-fence structure, so that the chambers are communicated with one another, and the chip is used for co-culture of different cells; every three adjacent chambers serve as a liver blood sinus unit and are respectively used for culturing parenchymal liver cells, hepatic stellate cells, endothelial cells and Kuptake cells, and the endothelial cells and the Kuptake cells are co-cultured in the same chamber. According to the method, four main cells related to hepatic fibrosis are introduced, corresponding stimulation on different cells in a time-sharing and zoning manner is supported, the construction speed and bionic property of an in-vitro hepatic fibrosis disease model are improved, and development and evaluation of hepatic fibrosis drugs and therapies are facilitated. 本发明公开了一种空间分辨型肝纤维化