Synthesis and application of superoxide anion free radical detection fluorescent probe based on silicon rhodamine

The invention discloses a fluorescent probe for detecting O < 2 >. The probe is constructed by taking silicon rhodamine as a fluorophore and taking trifluoromethanesulfonyl as a recognition group. In the presence of O2 , O2 can perform nucleophilic attack on sulfonate, so that quaternary ammonium salt is decomposed, and then silicon rhodamine luminophores are released and ring opening of the silicon rhodamine luminophores is promoted. Before the reaction, the silicon rhodamine quaternary ammonium salt 7 with the spiro structure is kept in a non-fluorescent state and has a low background; after the reaction, the free and ring-opened silicon rhodamine dye can be excited to emit near-infrared fluorescence, so that the aim of detecting O2 is fulfilled, and the method has a very good application prospect in the field of biological related science. The fluorescent probe for detecting O2 has a structure as shown in a formula 7: # imgabs0 #. 本发明公开了一种用于检测O2·-的荧光探针，该探针以硅罗丹明为荧光基团，三氟甲磺酰基为识别基团构建。在O2·-存在