Rapid mulberry breeding method

The invention discloses a rapid mulberry breeding method, and mainly relates to a breeding method. Comprising the following steps: S1, taking materials and inducing calluses; s2, culturing mulberry suspension cells; s3, performing enzymolysis to prepare protoplast; and S4, protoplast fusion. The met...

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Bibliographische Detailangaben
Hauptverfasser: TAN JING'AN, YANG YUBO, DAI YUXIN, WANG HOUWEI, WANG AIYUAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a rapid mulberry breeding method, and mainly relates to a breeding method. Comprising the following steps: S1, taking materials and inducing calluses; s2, culturing mulberry suspension cells; s3, performing enzymolysis to prepare protoplast; and S4, protoplast fusion. The method has the advantages that the method is simple, convenient and cheap, and field operation occupying a large amount of field manpower and material resources is completed in a laboratory. The method is rapid and efficient, and a large number of excellent genes of mulberry trees can be rapidly concentrated in one variety by utilizing a technology. The interference by various factors such as different karyotypes and chromosome characters is not easily caused. 本发明公开了一种桑树快速育种方法,主要涉及一种育种方法。包括以下步骤:S1,取材诱导愈伤组织;S2,培养桑树悬浮细胞;S3,酶解制成原生质体;S4,原生质体融合。本发明的有益效果在于:它简便便宜,将占用大量田地人力物力的田间操作在实验室里面完成。快速高效,可以利用技术将大量桑树的优良基因快速集中于一种品种。不易受到核型不同以及及染色体性状等各类因素干扰。