Method for detecting N-nitrosonorminocyclin-1 in medicine

The invention provides a method for detecting N-nitroso-demethylated minocyclin-1 in a medicine, which comprises the following steps: (1) preparation of a test solution: taking a test, and adding a diluent to prepare the test solution; (2) preparation of a reference substance solution: taking an N-nitroso-demethylated minocyclin-1 reference substance, and adding a diluent to prepare the reference substance solution in a linear concentration range; and (3) detecting the test solution and the reference substance solution by adopting an ultra-high performance liquid chromatography-mass spectrometer, and calculating the content of the N-nitrosonorminocyclin-1 in the test solution according to an external standard method. The method disclosed by the invention is simple in sample pretreatment, high in sensitivity, strong in specificity, good in linearity and durability and accurate in quantification. 本发明提供了一种药物中N-亚硝基去甲基米诺环素-1的检测方法，该方法包括：(1)供试品溶液的制备：取供试品，加入稀释剂配制成供试品溶液；(2)对照品溶液的制备：取N-亚硝基去甲基米诺环素-1对照品，加稀释剂配制成线性浓度范围的对照品