Detection kit for IDH1 and IDH2 gene mutation

The invention discloses a detection kit for IDH1 and IDH2 gene mutation. Aiming at 11 mutation types of IIDH1 and IDH2 genes, an LNA probe with high affinity and specificity is combined with a multiple TaqMan fluorescent probe, and a triple fluorescent PCR and digital PCR detection system capable of effectively detecting and quantifying IDH1R132 mutation and IDH2R172 mutation in the same tube reaction at the same time is established. The detection system comprises multiple IDH mutation cancer species and is suitable for detection of multiple types of clinical samples, especially the characteristics of high sensitivity and accuracy of digital PCR, so that tumor noninvasive diagnosis and real-time curative effect monitoring based on gene mutation content change analysis in a body fluid sample of a tumor patient become possible, and therefore, the detection system has a good application prospect. 本发明公开了IDH1和IDH2基因突变的检测试剂盒。本发明针对IIDH1和IDH2基因的11种突变类型，将高亲和力和特异性的LNA探针和多重TaqMan荧光探针相结合，建立了可在同一管反应内同时有效检测和定量IDH1R132突变和ID