Synthesis method of nucleic acid with polyA sequence

The invention relates to a synthesis method of nucleic acid with a polyA sequence. According to the synthetic method of the nucleic acid with the polyA sequence, the synthesis of the polyA sequence does not depend on Oligo annealing any more, a super-long primer is artificially synthesized by a chemical method aiming at the full-length polyA sequence, and the full length of the nucleic acid with the polyA sequence can be obtained by only performing a round of PCR in the whole synthetic process, so that the experimental operation is greatly simplified, the tedious experimental steps in Oligo annealing are avoided, and the yield of the nucleic acid with the polyA sequence is improved. And the time cost is reduced. 本发明涉及一种具有polyA序列的核酸的合成方法。该具有polyA序列的核酸的合成方法，对于polyA序列的合成不再依赖Oligo退火，针对全长polyA序列使用化学方法人工合成超长引物，整个合成过程只需要进行一轮PCR即可获得具有polyA序列的核酸全长，大大简化了实验操作，规避了Oligo退火中繁琐的实验步骤，降低了时间成本。