Research on treatment of brain glioma by inhibiting cell EMT through multi-level chitosan gelatin particle-loaded P4HA1 siRNA

The invention discloses a research on treatment of brain glioma by inhibiting cell EMT (epidermal growth factor) through multi-level chitosan gelatin particle-loaded P4HA1siRNA, and belongs to the field of biological medicine. Chitosan serves as a basic carrier and is compounded with gelatin to form a scaffold material, a P4HA1 gene serves as a target gene, and it is observed in vitro that chitosan gelatin particle loaded P4HA1siRNA inhibits cell EMT brain glioma proliferation and migration, so that the effect of effectively treating brain glioma is achieved. Meanwhile, the chitosan gelatin microsphere labeled 125I is subjected to in-vivo imaging, and the in-vivo biological distribution and the tumor suppression effect of the chitosan gelatin microsphere loaded P4HA1siRNA are observed and evaluated. 本发明公开了多层级壳聚糖明胶颗粒载P4HA1siRNA通过抑制细胞EMT治疗脑胶质瘤的研究，属于生物医药领域。本发明将壳聚糖作为基础载体，与明胶复合形成支架材料，以P4HA1基因作为目的基因，在体外观察壳聚糖明胶颗粒载P4HA1siRNA通过抑制细胞EMT脑胶质瘤增殖，迁移，达到对脑胶质瘤有效治疗的效果。同时将壳聚糖明胶微球标记125I进行体内成像，观察和评价壳聚糖明胶微球载P4HA1siRNA的体内生物分布和抑瘤效果。