Display substrate, preparation method thereof and display device

The invention provides a display substrate. The display substrate comprises a substrate body; the plurality of first electrode parts are positioned on the substrate; the plurality of light-emitting function parts are located on the sides, away from the substrate, of the plurality of first electrode parts; the pixel defining layer is located on the sides, away from the substrate, of the multiple first electrode parts, the pixel defining layer comprises multiple openings, in at least part of the first electrode parts, at least one groove is formed in the first electrode parts, and the grooves extend along the edges of the first electrode parts; the distance between the orthographic projection of the side wall, close to the edge of the first electrode part, of the groove on the substrate and the edge of the orthographic projection of the first electrode part on the substrate is smaller than or equal to a specified distance; the light-emitting function part comprises a first part and a second part, the first part