Display substrate, manufacturing method thereof and display device

The invention provides a display substrate, a manufacturing method thereof and a display device, relates to the technical field of display, and solves the problem that an existing display product cannot be well compatible with light extraction efficiency and low power consumption. The display substrate comprises a substrate body and sub-pixels arranged on the substrate body, each sub-pixel comprises a first light-emitting element and a second light-emitting element which are arranged in a stacked mode, and the first light-emitting elements are located between the second light-emitting elements and the substrate body; the first light-emitting element comprises a first electrode, a first light-emitting functional layer and a second electrode which are sequentially stacked in the direction away from the substrate, and the second light-emitting element comprises a third electrode, a second light-emitting functional layer and a fourth electrode which are sequentially stacked in the direction away from the substrat