Preparation method and application of biosensor of diabetic nephropathy marker

The invention relates to the technical field of two-dimensional material biological detection, in particular to a preparation method and application of a biosensor of a diabetic nephropathy marker. The two-dimensional tungsten sulfide crystal is used as an electric sensitive layer material, and the material can change more sensitively under the action of an external electric field, so that SPR signal adjustment is realized. The invention discloses a method for preparing a high-quality single-atomic-layer tungsten sulfide crystal, successfully designs an SPR biosensor by adopting the single-layer tungsten sulfide crystal as an electric sensitive layer, achieves a lower detection limit on MCP-1 protein, and realizes ultrahigh-sensitivity detection of a clinical sample. 本申请涉及二维材料生物检测技术领域，更具体地说，它涉及一种糖尿病肾病标志物的生物传感器的制备方法及其4应用。利用二维硫化钨晶体作为电敏感层材料，该材料在外加电场的作用下会发生更敏感的变化从而实现SPR信号的调节。制备高质量单原子层硫化钨晶体的方法，并且成功设计了采用单层硫化钨晶体作为电敏感层成功设计了SPR生物传感器，对MCP-1蛋白达到了较低的检测极限，并实现了临床样本的超高灵敏度检测。