Method for simultaneously detecting oxamacycline and tigecycline in blood through high performance liquid chromatography-tandem mass spectrometry and application of method for simultaneously detecting oxamacycline and tigecycline in blood

The invention provides a method for simultaneously detecting oxamacycline and tigecycline in blood through high performance liquid chromatography-tandem mass spectrometry and application, and relates to the technical field of medicine detection. The method for simultaneously detecting the oxamacycline and the tigecycline in the blood by the high performance liquid chromatography-tandem mass spectrometry comprises the following steps: preparing a standard curve equation, processing to obtain a sample to be detected, and detecting the sample to be detected. According to the method disclosed by the invention, tetracycline is used as an internal standard substance, and a method for simultaneously detecting after mixing standard substances is adopted, so that the detection efficiency of the oxalicycline and the tigecycline is effectively improved. The detection method disclosed by the invention is good in reproducibility and high in extraction recovery rate, and the accuracy of a detection result is improved. The