Wheat gene homozygosity detection method

The invention discloses a wheat gene homozygous detection method, which is characterized in that the relative copy number of a target gene is detected by using quantitative PCR (Polymerase Chain Reaction) based on a universal two-color fluorescent probe, and whether the target gene exists and is homozygous is determined, the detection method is not influenced by allelic variation corresponding to the target gene, and the detection accuracy is high. The defect that some developed KASP markers are difficult to distinguish homozygosis and heterozygosis is overcome, and the marker is suitable for carrying out molecular marker-assisted hybridization transformation on deletion sites. 本发明公开了一种小麦基因纯合性的检测方法，利用基于通用双色荧光探针的定量PCR来检测目的基因的相对拷贝数，并由此确定目的基因是否存在以及是否纯合，此检测方法不受与目的基因对应的等位变异的影响，解决了某些已开发的KASP标记难以区分纯合与杂合的缺陷，适合对缺失位点进行分子标记辅助的杂交转育。