DNA enzyme-based fluorescent biosensor for detecting salmonella

The invention belongs to the technical field of biosensors, and provides a DNA enzyme-based fluorescent biosensor for detecting salmonella, which comprises an Apt-T composite probe, Apt, a T chain, DNA enzyme A2E, a fluorophore FAM modified Y3, a quenching group BHQ1 modified Y1 chain, a quenching g...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	LI BOWEN, ZHU RUI, XIAO YIJING, HUANG JIADONG, YANG MEILI, GUO ZHIQIANG, SUN WEIQING, WANG YU, LIU SU, PANG QING'AO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of biosensors, and provides a DNA enzyme-based fluorescent biosensor for detecting salmonella, which comprises an Apt-T composite probe, Apt, a T chain, DNA enzyme A2E, a fluorophore FAM modified Y3, a quenching group BHQ1 modified Y1 chain, a quenching group Y2 chain, a quenching group A1 chain and a quenching group H chain, the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the nucleotide sequences of the Apt chain, the T chain, the DNA enzyme A2E chain, the DNA enzyme Y3 chain, the DNA enzyme Y1 chain, the DNA enzyme Y2 chain, the DNA enzyme A1 chain and the DNA enzyme H chain are as shown in SEQ ID NO: 1-6. The sensor disclosed by the invention has the advantages of high detection speed, simplicity in operation, low price, low detection limit, high specificity and the like, can make up the defects and deficiencies of the existing detection method of salmonella, and realizes rapid and accurate quantitative detection of salmonella.