Escherichia coli detection kit based on combination of LAMP amplification and CRISPR/Cas detection

The invention discloses an Escherichia coli detection kit based on combination of LAMP (Loop-Mediated Isothermal Amplification) and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (Clustered Regularly Interspaced Short Palindromic Repeats) detection. The Escherichia coli detection kit comprises a specific amplification primer of an Escherichia coli ompF gene, sgRNA (Ribonucleic Acid) capable of guiding CRISPR/Cas protease to recognize a target sequence, DNA (Deoxyribose Nucleic Acid) polymerase, CRISPR/Cas protease and a fluorescent reporter probe. The invention also discloses a combination of the specific amplification primer and the sgRNA, and a method for detecting the Escherichia coli by using the kit. DNA polymerase and CRISPR/Cas protease obtained through expression and purification of ompF gene deleted escherichia coli are used as reaction enzymes, specific primers are designed for ompF gene sequences, specific recognition is enhanced by combining sgRNA, the accuracy of virus cha