Dual-luciferase reporter gene vector based on human LDHA gene promoter region as well as construction method and application of dual-luciferase reporter gene vector

The invention discloses a dual-luciferase reporter gene vector based on a human LDHA gene promoter region as well as a construction method and application of the dual-luciferase reporter gene vector. The dual-luciferase reporter gene vector is marked as pGL3-LDHA-promoter with a nucleotide sequence as shown in SEQ ID NO.2. The dual-luciferase reporter gene vector is constructed by utilizing an efficient enzyme and a homologous recombination principle. BgL II and HindIII are utilized to complete cloning and recombination of an LDHA gene promoter target fragment and a pGL3-Basic linearized vector, and a double enzyme digestion method can greatly reduce self-connection of the vector and improve the positive rate of the recombinant vector. The pGL3-LDHA-promoter can be used for realizing sensitive identification and accurate detection on the expression quantity of the LDHA promoter. 本发明公开了一种基于人LDHA基因启动子区的双荧光素酶报告基因载体及其构建方法和应用，该双荧光素酶报告基因载体记为pGL3-LDHA-promoter核苷酸序列如SEQ ID NO.2所示，利用高效酶和同源重组原理，利用BgL II以及HindⅢ完成了LDHA基因