Bacillus licheniformis for integratively expressing phospholipase D as well as construction method and application of bacillus licheniformis

The invention relates to the technical field of gene engineering, in particular to bacillus licheniformis for integrative expression of phospholipase D as well as a construction method and application of the bacillus licheniformis. Phospholipase D is integrated into a bacillus licheniformis genome,...

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Bibliographische Detailangaben
Hauptverfasser:	SUN JUN, YANG XIANGMEI, XING CHENGUANG, LIU GANG, DAI HUILING, LIN XILIAN, GAO ZEPAN, YUE XIA, DENG DONGLIAN, JEONG DONG-WOOK
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of gene engineering, in particular to bacillus licheniformis for integrative expression of phospholipase D as well as a construction method and application of the bacillus licheniformis. Phospholipase D is integrated into a bacillus licheniformis genome, recombinant bacteria are subjected to fed-batch fermentation culture for 72 hours, and the extracellular enzyme activity is 165.67 U/mL. The bacillus licheniformis recombinant strain provided by the invention can be passed for many times, and the enzyme production stability after passage is good; antibiotics are not needed for screening plasmids, the enzyme does not need to be expressed by adding an inducer, and the recombinant phospholipase D can be directly applied to synthesis of food-grade products and has an industrial application prospect. 本发明涉及基因工程技术领域，具体涉及一种整合型表达磷脂酶D的地衣芽孢杆菌及其构建方法、应用。本发明将磷脂酶D整合入地衣芽孢杆菌基因组，重组菌经过72小时补料发酵培养，胞外酶活为165.67U/mL。本发明提供的地衣芽孢杆菌重组菌可传代次数多，传代后产酶稳定性好；不需要抗生素筛选质粒，酶不需要添加诱导剂表达，重组的磷脂酶D可以直接应用于食品级产