Hybridoma cell secreting KOD DNA polymerase monoclonal antibody, monoclonal antibody and application of monoclonal antibody

The invention relates to the technical field of biology, in particular to a hybridoma cell capable of secreting a KOD DNA polymerase monoclonal antibody, the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody which can be specifically combined with KOD DNA polymerase is produced by using hybridoma cells, and the KOD DNA polymerase can be released under the condition of thermal activation, so that polymerization and excision functions of the KOD DNA polymerase are recovered. The monoclonal antibody can be widely applied to PCR amplification, DNA sequencing, gene cloning, protein expression, protein purification and other technologies in the field of molecular biology. The monoclonal antibody can also be used for diagnosis, treatment, prevention and the like in the field of biomedicine, such as detection of pathogenic microorganisms, tumor markers, gene mutation and the like, and gene therapy, protein therapy and the like for treating some diseases, such as synthesis of a cD