Polypeptide specifically bound with triple negative breast cancer cells and application thereof

The invention relates to the technical field of biology, in particular to polypeptide specifically bound with triple-negative breast cancer cells and application of the polypeptide. A phage display technology is adopted, normal breast cell strain MCF 10A cells serve as adsorption cells, triple-negative breast cancer cell strain MDA-MB-231 cells serve as target cells, several rounds of subtraction screening are carried out, and the polypeptide specifically bound with triple-negative breast cancer cells is obtained. Obtaining positive bacteriophage clone which is specifically combined with the MDA-MB-231 cell; the method comprises the following steps of: randomly selecting positive bacteriophage clones for DNA sequencing, deducing an amino acid sequence of a corresponding display peptide according to an inserted DNA base sequence displayed by a sequencing result, and screening and sequencing to finally obtain a polypeptide amino acid series, namely Phe-Arg-Ala-Met-Gly-Val-Ser, which is specifically combined wit