Closing sequence and kit and use method thereof

The invention discloses a closed sequence and a kit and a use method thereof. The closing sequence comprises a closing sequence 1, a closing sequence 2, a closing sequence 3 and a closing sequence 4; the closed sequence 1 is complementary to the sequence of the sequencing linker P5 and is used for c...

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Bibliographische Detailangaben
1. Verfasser:	YE BANGQUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a closed sequence and a kit and a use method thereof. The closing sequence comprises a closing sequence 1, a closing sequence 2, a closing sequence 3 and a closing sequence 4; the closed sequence 1 is complementary to the sequence of the sequencing linker P5 and is used for closing the sequence of the sequencing linker P5 at the 5'end of the first chain when the target area capture library is prepared; the closed sequence 2 is complementary to the Rsp1 sequence and is used for closing the Rsp1 sequence in the direction from the 5'end to the 3 'end on the first chain during preparation of the target area capture library; the closed sequence 3 and the Rsp2'sequence are partially complementary, and the closed sequence 3 is used for closing the Rsp2 'sequence in the direction from the 5'end to the 3' end on the second chain when the target area capture library is prepared; the closing sequence 4 is complementary with the sequence of the sequencing linker P7'and is used for closing the sequ