Mitochondrial DNA data normalization method and integrated application platform

The invention belongs to the field of bioinformatics and software engineering, and discloses a mitochondrial DNA data normalization method and an integrated application platform, and the method comprises the following steps: obtaining mitochondrial genome files of different types of species from a mitochondrial genome public database; the mitochondrial genome file is nested and traversed, annotation information and gene sequences of all species in the mitochondrial genome file are obtained, the mitochondrial genome file comprises gene information of multiple species, and each piece of gene information further comprises multiple pieces of annotation information; analyzing a gene type corresponding to each sequence coordinate, and appointing a replacement scheme according to the gene type; comparing adjacent genes and sequence coordinates of each gene; performing reverse complementation and slice zeroing, comparing the reference sequence version with an annotation version submitted by an original client, and li