Electrochemiluminescence detection kit for PEG-EPO content in human serum and application thereof

The invention discloses an electrochemiluminescence detection kit for PEG-EPO content in human serum and application of the electrochemiluminescence detection kit. Comprising an MSD plate, a coating antibody, a coating antibody diluent, a washing solution, a sealing working solution, a detection antibody, a detection antibody diluent, a second antibody working solution, Read Buffe and a PEG-EPO standard substance, the coating antibody is a polyethylene glycol mouse monoclonal antibody, and the detection antibody is a human erythropoietin antibody. According to the method, firstly, multiple anti-PEG antibodies and anti-EPO antibodies are selected for enzyme-linked immunosorbent assay antibody pair screening, the result shows that no one group of antibody pairs can meet the detection requirement, then, an electrochemical luminescence method is used for antibody pair screening, and one group of antibody pairs can well meet the sensitivity of method detection. The detection method can be used for quantitative det