Method for establishing HPLC (High Performance Liquid Chromatography) fingerprint spectrum of Yao medicine Indian neem and identifying basis

The invention discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a Yao medicine Indian nightshade root and an original identification method. The method comprises the steps of preparation of a test solution, preparation of a reference solution, chromatographic conditions and determination, the obtained HPLC fingerprint spectrum can effectively represent the chemical components and quality of the Yao medicine Indian nightshade root, and the method has the advantages of rapidness, accuracy, rich chromatographic peak information, good separation effect and the like. Through pharmacology and active ingredient research, the medicinal material source of the pubescent solomonseal rhizome is increased; the HPLC fingerprint spectrum base original identification method of the zanthoxylum nitidum, the zanthoxylum nitidum, the zanthoxylum armatum and the zanthoxylum armatum is established. According to the method, a scientific, comprehensive and accurate quality s