Construction method of medium-and-low-flux genome sample mixing library
The invention relates to the technical field of gene library construction, and particularly discloses a construction method of a medium-and-low-flux genome sample mixing library. The method comprises the following steps: preparing bar code labeled DNA: mixing and connecting a Tn5 transposase complex...
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| creator | WANG YUKUAN SUN LUYANG XU JIAN GE YUQIAN WANG AOHUA |
| description | The invention relates to the technical field of gene library construction, and particularly discloses a construction method of a medium-and-low-flux genome sample mixing library. The method comprises the following steps: preparing bar code labeled DNA: mixing and connecting a Tn5 transposase complex and a DNA fragment to obtain a labeled broken product; connecting the tagged broken product with a connector, purifying the product, and amplifying and enriching the product to obtain bar code tagged DNA (Deoxyribose Nucleic Acid); according to the method, different bar code labeled DNAs are respectively prepared by taking different DNA fragments as raw materials, and different bar code sequences are contained in the different bar code labeled DNAs; and library mixing and purification: mixing and purifying different bar code labeled DNAs to obtain a mixed sample library. The method provided by the invention can realize library construction and detection with low cost and high coverage.
本申请涉及基因文库构建的技术领域，具体公开了一种中低通量 |
| format | Patent |
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本申请涉及基因文库构建的技术领域，具体公开了一种中低通量</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMBINATORIAL CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240702&DB=EPODOC&CC=CN&NR=118272933A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240702&DB=EPODOC&CC=CN&NR=118272933A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG YUKUAN</creatorcontrib><creatorcontrib>SUN LUYANG</creatorcontrib><creatorcontrib>XU JIAN</creatorcontrib><creatorcontrib>GE YUQIAN</creatorcontrib><creatorcontrib>WANG AOHUA</creatorcontrib><title>Construction method of medium-and-low-flux genome sample mixing library</title><description>The invention relates to the technical field of gene library construction, and particularly discloses a construction method of a medium-and-low-flux genome sample mixing library. The method comprises the following steps: preparing bar code labeled DNA: mixing and connecting a Tn5 transposase complex and a DNA fragment to obtain a labeled broken product; connecting the tagged broken product with a connector, purifying the product, and amplifying and enriching the product to obtain bar code tagged DNA (Deoxyribose Nucleic Acid); according to the method, different bar code labeled DNAs are respectively prepared by taking different DNA fragments as raw materials, and different bar code sequences are contained in the different bar code labeled DNAs; and library mixing and purification: mixing and purifying different bar code labeled DNAs to obtain a mixed sample library. The method provided by the invention can realize library construction and detection with low cost and high coverage.
本申请涉及基因文库构建的技术领域，具体公开了一种中低通量</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMBINATORIAL CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHB3zs8rLikqTS7JzM9TyE0tychPUchPA7JSMktzdRPzUnRz8st103JKKxTSU_Pyc1MVihNzC3JSFXIzKzLz0hVyMpOKEosqeRhY0xJzilN5oTQ3g6Kba4izh25qQX58anFBYnJqXmpJvLOfoaGFkbmRpbGxozExagCRxjRB</recordid><startdate>20240702</startdate><enddate>20240702</enddate><creator>WANG YUKUAN</creator><creator>SUN LUYANG</creator><creator>XU JIAN</creator><creator>GE YUQIAN</creator><creator>WANG AOHUA</creator><scope>EVB</scope></search><sort><creationdate>20240702</creationdate><title>Construction method of medium-and-low-flux genome sample mixing library</title><author>WANG YUKUAN ; SUN LUYANG ; XU JIAN ; GE YUQIAN ; WANG AOHUA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118272933A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMBINATORIAL CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG YUKUAN</creatorcontrib><creatorcontrib>SUN LUYANG</creatorcontrib><creatorcontrib>XU JIAN</creatorcontrib><creatorcontrib>GE YUQIAN</creatorcontrib><creatorcontrib>WANG AOHUA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG YUKUAN</au><au>SUN LUYANG</au><au>XU JIAN</au><au>GE YUQIAN</au><au>WANG AOHUA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Construction method of medium-and-low-flux genome sample mixing library</title><date>2024-07-02</date><risdate>2024</risdate><abstract>The invention relates to the technical field of gene library construction, and particularly discloses a construction method of a medium-and-low-flux genome sample mixing library. The method comprises the following steps: preparing bar code labeled DNA: mixing and connecting a Tn5 transposase complex and a DNA fragment to obtain a labeled broken product; connecting the tagged broken product with a connector, purifying the product, and amplifying and enriching the product to obtain bar code tagged DNA (Deoxyribose Nucleic Acid); according to the method, different bar code labeled DNAs are respectively prepared by taking different DNA fragments as raw materials, and different bar code sequences are contained in the different bar code labeled DNAs; and library mixing and purification: mixing and purifying different bar code labeled DNAs to obtain a mixed sample library. The method provided by the invention can realize library construction and detection with low cost and high coverage.
本申请涉及基因文库构建的技术领域，具体公开了一种中低通量</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Construction method of medium-and-low-flux genome sample mixing library |
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