Construction method of medium-and-low-flux genome sample mixing library

The invention relates to the technical field of gene library construction, and particularly discloses a construction method of a medium-and-low-flux genome sample mixing library. The method comprises the following steps: preparing bar code labeled DNA: mixing and connecting a Tn5 transposase complex and a DNA fragment to obtain a labeled broken product; connecting the tagged broken product with a connector, purifying the product, and amplifying and enriching the product to obtain bar code tagged DNA (Deoxyribose Nucleic Acid); according to the method, different bar code labeled DNAs are respectively prepared by taking different DNA fragments as raw materials, and different bar code sequences are contained in the different bar code labeled DNAs; and library mixing and purification: mixing and purifying different bar code labeled DNAs to obtain a mixed sample library. The method provided by the invention can realize library construction and detection with low cost and high coverage. 本申请涉及基因文库构建的技术领域，具体公开了一种中低通量