Molecular marker primer of white gourd fusarium wilt resistant major gene Fob1 and marking method and application thereof

The invention relates to the technical field of molecular genetics, in particular to a molecular marker primer of a white gourd fusarium wilt resistant major gene Fob1 and a marking method and application of the molecular marker primer. According to the molecular marker primer, a high white gourd fusarium wilt resistant material GD68 and a high fusarium wilt susceptible material HM25 are hybridized to obtain an F1 heterozygous seed, and then selfing is conducted to a first generation to obtain an F2 genetic population; genetic linkage analysis is carried out through the genotype of each single plant of the F2 group and the phenotype of the disease resistance level of fusarium wilt to identify that the white gourd material GD68 carries a disease-resistant major gene, and the white gourd material GD68 is named as Fob1. Molecular markers 3M9.425, 3M13.385 and 3M16.869 are developed according to the major gene, the molecular markers can effectively detect whether the major resistance gene is carried in a white go