Method for constructing polycomb protein complex of multi-genome assembly

The invention provides a construction method of a polycomb protein complex of a multi-genome package. The construction method comprises the following steps: assembling and constructing a PRC2 complex strain containing 10 genes, assembling and constructing a Backup complex strain containing 6 genes, hybridizing the two constructed and assembled complex strains, and the like. The problems that in the prior art, protein complex construction is complex, and the number of gene co-expression is small are solved, the DNA assembly technology and the yeast hybridization technology are combined, the efficiency of in-vitro protein complex construction is improved, and meanwhile co-expression of multiple genes is greatly increased. 本发明提供了一种多基因组装的polycomb蛋白复合体的构建方法，包括组装构建含有10个基因的PRC2复合体菌株、组装构建含有6个基因的Backup复合体菌株以及将构建组装好的两种复合体菌株进行杂交等步骤。本发明解决了现有技术中存在蛋白复合体构建复杂且基因共表达数量少的问题，本发明结合DNA组装技术和酵母杂交技术，在提高了体外构建蛋白复合体的效率的同时，并且极大的增加了多个基因的共表达。